Hotspot

orf621

Genome · 106086-108002 · 1917 bp

Back to Campylaephora

Pi 0.113

MarkerSeek score 77.90

Primer available yes

Label orf621

Feature Metadata

label_name: orf621
feature_type: gene
region: Genome
length_bp: 1917
variable_sites: 216
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf621_p1	CCAAGTTCCTTCAATGTCATGC	CCAGCCATGTGGAGAATGAA	45.5	50.0	58.2	57.9	2720 bp	0.5	41.7
orf621_p2	CAAGTTCCTTCAATGTCATGCA	CCAGCCATGTGGAGAATGAA	40.9	50.0	57.5	57.9	2719 bp	0.5	38.9
orf621_p3	CCAAGTTCCTTCAATGTCATGC	GAACGCGGTATAACTCCTGT	45.5	50.0	58.2	57.4	2131 bp	0.5	38.1
orf621_p4	AGTTCCTTCAATGTCATGCA	CCAGCCATGTGGAGAATGAA	40.0	50.0	55.2	57.9	2717 bp	0.5	36.9
orf621_p5	CCAAGTTCCTTCAATGTCATGC	AGAACGCGGTATAACTCCTG	45.5	50.0	58.2	57.1	2132 bp	0.5	35.8