Hotspot

QQR83_pgp086

Genome · 103059-103838 · 780 bp

Back to Campylaephora

Pi 0.0962

MarkerSeek score 78.90

Primer available yes

Label QQR83_pgp086

Feature Metadata

label_name: QQR83_pgp086
feature_type: gene
region: Genome
length_bp: 780
variable_sites: 75
pi: 0.0962
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
QQR83_pgp086_p1	ACCAACTTCAGCAAGCTCTT	TGCGTATACCAATTTCGCCA	45.0	45.0	57.6	58.0	1435 bp	1.0	82.9
QQR83_pgp086_p2	ACCAACTTCAGCAAGCTCTT	CCAATTTCGCCATCTACCCA	45.0	50.0	57.6	57.9	1427 bp	1.0	82.7
QQR83_pgp086_p3	ACCAACTTCAGCAAGCTCTT	ACCAATTTCGCCATCTACCC	45.0	50.0	57.6	57.9	1428 bp	1.0	82.7
QQR83_pgp086_p4	ACCAACTTCAGCAAGCTCTT	TGCTCGTCTTACAATCTGCC	45.0	50.0	57.6	58.0	1603 bp	0.5	57.1
QQR83_pgp086_p5	GCTGCAACAACTGACCCTAA	TGCTCGTCTTACAATCTGCC	50.0	50.0	58.4	58.0	1683 bp	0.5	56.8