Hotspot

QQR83_pgt016-QQR83_pgt017

Genome · 110740-110756 · 17 bp

Back to Campylaephora

Pi 0.176

MarkerSeek score 77.80

Primer available yes

Label QQR83_pgt016-QQR83_pgt017

Feature Metadata

label_name: QQR83_pgt016-QQR83_pgt017
feature_type: igs
region: Genome
length_bp: 17
variable_sites: 3
pi: 0.176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
QQR83_pgt016-QQR83_pgt017_p1	AATCCGCCATCCTGAAAAGG	TTCCAAGCTAATGATGCGGG	50.0	50.0	58.2	58.3	107 bp	1.0	77.8
QQR83_pgt016-QQR83_pgt017_p2	AATCCGCCATCCTGAAAAGG	GCTAATGATGCGGGTTCGAT	50.0	50.0	58.2	58.5	101 bp	1.0	76.6
QQR83_pgt016-QQR83_pgt017_p3	AATCCGCCATCCTGAAAAGG	CCAAGCTAATGATGCGGGTT	50.0	50.0	58.2	58.6	105 bp	1.0	75.6
QQR83_pgt016-QQR83_pgt017_p4	CCATCCTGAAAAGGACACCG	TTCCAAGCTAATGATGCGGG	55.0	50.0	58.5	58.3	101 bp	1.0	75.4
QQR83_pgt016-QQR83_pgt017_p5	AATCCGCCATCCTGAAAAGG	AATGATGCGGGTTCGATTCC	50.0	50.0	58.2	58.7	98 bp	1.0	75.1