Hotspot

rps10-tufA

Genome · 39283-39302 · 20 bp

Back to Campylaephora

Pi 0.105

MarkerSeek score 77.80

Primer available yes

Label rps10-tufA

Feature Metadata

label_name: rps10-tufA
feature_type: igs
region: Genome
length_bp: 20
variable_sites: 2
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps10-tufA_p1	AGATGTTGCAGAAGACGAGG	TGTTTCTGGTTCAGCCCTTC	50.0	50.0	57.6	58.0	1337 bp	0.5	56.8
rps10-tufA_p2	AGATGTTGCAGAAGACGAGG	AACTGTTGGAGCAGGAGTTG	50.0	50.0	57.6	58.0	656 bp	0.5	56.8
rps10-tufA_p3	AGATGTTGCAGAAGACGAGG	CTGTTGGAGCAGGAGTTGTT	50.0	50.0	57.6	58.0	654 bp	0.5	56.8
rps10-tufA_p4	AGATGTTGCAGAAGACGAGG	ACTGTTGCAACTGGTCGAAT	50.0	45.0	57.6	58.0	1141 bp	0.5	56.7
rps10-tufA_p5	AGATGTTGCAGAAGACGAGG	GGAGGAAGAACTGTTGGAGC	50.0	55.0	57.6	58.2	664 bp	0.5	55.5