Hotspot

rpl19-QQR83_pgt008

Genome · 29226-29442 · 217 bp

Back to Campylaephora

Pi 0.153

MarkerSeek score 77.50

Primer available yes

Label rpl19-QQR83_pgt008

Feature Metadata

label_name: rpl19-QQR83_pgt008
feature_type: igs
region: Genome
length_bp: 217
variable_sites: 31
pi: 0.153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl19-QQR83_pgt008_p1	AGCTTCTTCTTGTGCAAGCA	TCAGTGGTGATGCAAGCTTT	45.0	45.0	58.3	58.0	1311-1316 bp	1.0	84.7
rpl19-QQR83_pgt008_p2	AGCTTCTTCTTGTGCAAGCA	AGTGGTGATGCAAGCTTTCA	45.0	45.0	58.3	58.0	1309-1314 bp	1.0	84.6
rpl19-QQR83_pgt008_p3	AGCTTCTTCTTGTGCAAGCA	TGGTTGCACCAGGAAAAAGT	45.0	45.0	58.3	58.1	984-989 bp	1.0	84.6
rpl19-QQR83_pgt008_p4	AGCTTCTTCTTGTGCAAGCA	TACCGGCTGCAATAGGAGTA	45.0	50.0	58.3	57.9	1365-1370 bp	1.0	84.1
rpl19-QQR83_pgt008_p5	AGCTTCTTCTTGTGCAAGCA	TGCTGGCTTAGGAACAATGG	45.0	50.0	58.3	58.2	1394-1399 bp	1.0	83.4