Hotspot

ycf1

SSC · 109677-115157 · 5481 bp

Back to Campsis

Pi 0.373

MarkerSeek score 74.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5481
variable_sites: 1757
pi: 0.373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGAAGCACCAAACCAATCA	ACATACTGAAACGACTGCCA	45.0	45.0	57.6	57.2	415 bp	0.5	50.3
ycf1_p2	AAGAGGAAGCACCAAACCAA	ACATACTGAAACGACTGCCA	45.0	45.0	57.5	57.2	418 bp	0.5	49.7
ycf1_p3	CATTCGTCTGTCTGTCCCAA	ACATACTGAAACGACTGCCA	50.0	45.0	57.5	57.2	488 bp	0.5	49.7
ycf1_p4	AACCCATTCGTCTGTCTGTC	ACATACTGAAACGACTGCCA	50.0	45.0	57.5	57.2	492 bp	0.5	49.7
ycf1_p5	GAACCCATTCGTCTGTCTGT	ACATACTGAAACGACTGCCA	50.0	45.0	57.5	57.2	493 bp	0.5	49.7