Hotspot

ndhA

SSC · 117102-119284 · 2183 bp

Back to Campsis

Pi 0.462

MarkerSeek score 76.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2183
variable_sites: 756
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CAGGCTCTGGAAGGAATTCC	TTCCAATCAACAACCGGGAG	55.0	50.0	58.0	58.1	2868 bp	0.5	58.8
ndhA_p2	CAGGCTCTGGAAGGAATTCC	TGGCAGCTCGTATTGTTTGT	55.0	45.0	58.0	58.1	2675 bp	0.5	58.7
ndhA_p3	CAGGCTCTGGAAGGAATTCC	CCAATCAACAACCGGGAGAT	55.0	50.0	58.0	57.9	2866 bp	0.5	58.6
ndhA_p4	AGAGTCGAAGCACCAAAAGG	TTCCAATCAACAACCGGGAG	50.0	50.0	58.1	58.1	2712 bp	0.5	58.3
ndhA_p5	AGTCGAAGCACCAAAAGGAG	TTCCAATCAACAACCGGGAG	50.0	50.0	58.1	58.1	2710 bp	0.5	58.3