Hotspot

ndhD-ccsA

SSC · 123401-123648 · 248 bp

Back to Campsis

Pi 0.45

MarkerSeek score 76.70

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 248
variable_sites: 103
pi: 0.45
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TCAGTAGCTTTTCGATGGCC	CCGCCCTTTTATGTGGATCA	50.0	50.0	58.0	57.9	1155 bp	0.5	59.4
ndhD-ccsA_p2	CAGTAGCTTTTCGATGGCCT	CCGCCCTTTTATGTGGATCA	50.0	50.0	58.0	57.9	1154 bp	0.5	59.4
ndhD-ccsA_p3	TTGGTTGATGATGGTAGGCG	CCGCCCTTTTATGTGGATCA	50.0	50.0	58.0	57.9	1454 bp	0.5	59.3
ndhD-ccsA_p4	TCAGTAGCTTTTCGATGGCC	TTGGAGTTGGGACCCAAAAG	50.0	50.0	58.0	57.9	833 bp	0.5	59.2
ndhD-ccsA_p5	CAGTAGCTTTTCGATGGCCT	TTGGAGTTGGGACCCAAAAG	50.0	50.0	58.0	57.9	832 bp	0.5	59.1