Hotspot

ycf1-rps15

SSC · 115158-115549 · 392 bp

Back to Campsis

Pi 0.46

MarkerSeek score 77.40

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 392
variable_sites: 159
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	AGCAGCCAAGTTTCACATGA	CAGCAGTCGTTGACGTTTTC	45.0	50.0	58.0	58.0	788 bp	0.5	59.8
ycf1-rps15_p2	AGCAGCCAAGTTTCACATGA	TCCCAACGCGTTACATAAGG	45.0	50.0	58.0	58.0	1177 bp	0.5	59.8
ycf1-rps15_p3	AGCAGCCAAGTTTCACATGA	AGTCGTTGACGTTTTCCCAA	45.0	45.0	58.0	58.0	784 bp	0.5	59.7
ycf1-rps15_p4	AGCAGCCAAGTTTCACATGA	CCGAGTCATTCTTCCATGCA	45.0	50.0	58.0	58.0	956 bp	0.5	59.7
ycf1-rps15_p5	GCAGCCAAGTTTCACATGAC	CAGCAGTCGTTGACGTTTTC	50.0	50.0	57.9	58.0	787 bp	0.5	59.5