Hotspot

trnT-UGU-trnL-UAA

LSC · 46770-47506 · 737 bp

Back to Campsis

Pi 0.0408

MarkerSeek score 77.00

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 737
variable_sites: 29
pi: 0.0408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	797-798 bp	1.0	82.6
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	829-830 bp	1.0	81.5
trnT-UGU-trnL-UAA_p3	AAAATAGGAAAAGGCCGGCT	CCGTAGCGTCTACCGATTTC	45.0	55.0	57.8	58.2	852-853 bp	1.0	81.4
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	791-792 bp	1.0	81.3
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	829-830 bp	1.0	80.3