Hotspot

ycf2

LSC · 89838-96773 · 6936 bp

Back to Camphora

Pi 0.00548

MarkerSeek score 44.30

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6936
variable_sites: 38
pi: 0.00548
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TTGAGTCACAGGTATCTGGC	ACGGAGATAGTGATGCAAGT	50.0	45.0	56.9	56.3	254 bp	1.0	60.5
ycf2_p2	TTGAGTCACAGGTATCTGGC	TGAAACGGAGATAGTGATGCA	50.0	42.9	56.9	57.1	258 bp	1.0	58.6
ycf2_p3	TTGAGTCACAGGTATCTGGC	AACGGAGATAGTGATGCAAGT	50.0	42.9	56.9	57.1	255 bp	1.0	58.6
ycf2_p4	TTGAGTCACAGGTATCTGGC	ACGGAGATAGTGATGCAAGTT	50.0	42.9	56.9	57.1	254 bp	1.0	58.6
ycf2_p5	TCACCAATTGAGTCACAGGT	ACGGAGATAGTGATGCAAGT	45.0	45.0	56.7	56.3	261 bp	1.0	58.4