Hotspot

ycf1

SSC · 128406-134021 · 5616 bp

Back to Camphora

Pi 0.00893

MarkerSeek score 48.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5616
variable_sites: 50
pi: 0.00893
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAATCCCTTCTTTGTGAATGT	AGATCGGAATCGCAGAAACA	36.4	45.0	55.9	57.3	218 bp	1.0	43.6
ycf1_p2	TCATCAATCCCTTCTTTGTGA	AGATCGGAATCGCAGAAACA	38.1	45.0	54.8	57.3	221 bp	1.0	43.0
ycf1_p3	TCAAGGAAAAGTTGTACGTTG	AGATCGGAATCGCAGAAACA	38.1	45.0	54.7	57.3	170 bp	1.0	42.4
ycf1_p4	TGATTCAAGGAAAAGTTGTACGT	AGATCGGAATCGCAGAAACA	34.8	45.0	56.7	57.3	174 bp	1.0	42.3
ycf1_p5	CATCAATCCCTTCTTTGTGAATGT	AGATCGGAATCGCAGAAACA	37.5	45.0	57.7	57.3	220 bp	1.0	42.2