Hotspot

rpl32-trnL(TAG)

SSC · 117403-118792 · 1390 bp

Back to Camphora

Pi 0.0138

MarkerSeek score 64.30

Primer available yes

Label rpl32-trnL(TAG)

Feature Metadata

label_name: rpl32-trnL(TAG)
feature_type: igs
region: SSC
length_bp: 1390
variable_sites: 19
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(TAG)_p1	CGCGGCAAAAGCTTTATCTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.7	57.9	1523-1524 bp	1.0	78.8
rpl32-trnL(TAG)_p2	CGCGGCAAAAGCTTTATCTT	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.7	57.8	1516-1517 bp	1.0	78.3
rpl32-trnL(TAG)_p3	CGCGGCAAAAGCTTTATCTT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.7	57.8	1522-1523 bp	1.0	78.2
rpl32-trnL(TAG)_p4	CGCGGCAAAAGCTTTATCTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.7	57.8	1521-1522 bp	1.0	78.2
rpl32-trnL(TAG)_p5	CGCGGCAAAAGCTTTATCTT	GAGCAGCGTGTCTACCAATT	45.0	50.0	57.7	58.3	1517-1518 bp	1.0	77.6