Hotspot

ndhF-rpl32

SSC · 115994-117228 · 1235 bp

Back to Camphora

Pi 0.0133

MarkerSeek score 67.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1235
variable_sites: 16
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACAAGCAGAAGTCCCAA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1317-1347 bp	1.0	80.4
ndhF-rpl32_p2	AGCAGAAGTCCCAACCCTAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1311-1341 bp	1.0	80.3
ndhF-rpl32_p3	AAGCAGAAGTCCCAACCCTA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.3	57.7	1312-1342 bp	1.0	78.5
ndhF-rpl32_p4	GGAACAAGCAGAAGTCCCAA	GGCCTGATATCCCCTTCCTT	50.0	55.0	58.0	58.5	1375-1405 bp	1.0	78.3
ndhF-rpl32_p5	AGCAGAAGTCCCAACCCTAT	GGCCTGATATCCCCTTCCTT	50.0	55.0	58.0	58.5	1369-1399 bp	1.0	78.2