Hotspot

rpoB-trnC(GCA)

LSC · 27874-28963 · 1090 bp

Back to Camphora

Pi 0.0046

MarkerSeek score 61.80

Primer available yes

Label rpoB-trnC(GCA)

Feature Metadata

label_name: rpoB-trnC(GCA)
feature_type: igs
region: LSC
length_bp: 1090
variable_sites: 5
pi: 0.0046
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC(GCA)_p1	ATTCCCTCATTTCCATCCCG	TTGTTAATCAGGCGACACCC	50.0	50.0	57.3	58.2	1223-1226 bp	1.0	73.8
rpoB-trnC(GCA)_p2	ATTCCCTCATTTCCATCCCG	CTGGGGAGAAAGGATTTGCA	50.0	50.0	57.3	57.8	1194-1197 bp	1.0	73.5
rpoB-trnC(GCA)_p3	ATTCCCTCATTTCCATCCCG	TGGGGAGAAAGGATTTGCAG	50.0	50.0	57.3	57.8	1193-1196 bp	1.0	73.5
rpoB-trnC(GCA)_p4	ATTCCCTCATTTCCATCCCG	AGAAAGGATTTGCAGTCCCC	50.0	50.0	57.3	57.8	1188-1191 bp	1.0	73.5
rpoB-trnC(GCA)_p5	ATTCCCTCATTTCCATCCCG	GGGGAGAAAGGATTTGCAGT	50.0	50.0	57.3	57.8	1192-1195 bp	1.0	73.5