Hotspot

psbA

LSC · 93-1154 · 1062 bp

Back to Campeiostachys

Pi 0.000628

MarkerSeek score 36.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000628
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGGGTATTTCTGTCTTTTCTTTCT	AATACGTGTGCTTGGGAGTC	33.3	50.0	56.2	57.9	1167 bp	1.0	40.1
psbA_p2	TGGGTATTTCTGTCTTTTCTTTCTT	AATACGTGTGCTTGGGAGTC	32.0	50.0	56.9	57.9	1167 bp	1.0	40.1
psbA_p3	TTGGGTATTTCTGTCTTTTCTTTCT	AATACGTGTGCTTGGGAGTC	32.0	50.0	56.9	57.9	1168 bp	1.0	40.1
psbA_p4	GGGTATTTCTGTCTTTTCTTTCTTCA	AATACGTGTGCTTGGGAGTC	34.6	50.0	57.8	57.9	1166 bp	1.0	40.1
psbA_p5	TGGGTATTTCTGTCTTTTCTTTCTTC	AATACGTGTGCTTGGGAGTC	34.6	50.0	57.8	57.9	1167 bp	1.0	40.1