Hotspot

rpl32-trnL-UAG

SSC · 104909-105610 · 702 bp

Back to Campeiostachys

Pi 0.0057

MarkerSeek score 59.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 702
variable_sites: 6
pi: 0.0057
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTCCAGCGGTAATGAGCATC	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	840-841 bp	1.0	80.3
rpl32-trnL-UAG_p2	TTCCAGCGGTAATGAGCATC	GGATTCGAACCGAGATGCTT	50.0	50.0	58.0	58.1	843-844 bp	1.0	79.8
rpl32-trnL-UAG_p3	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	812-813 bp	1.0	79.6
rpl32-trnL-UAG_p4	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	811-812 bp	1.0	79.6
rpl32-trnL-UAG_p5	TTCCAGCGGTAATGAGCATC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	813-814 bp	1.0	79.3