Hotspot

ndhF-rpl32

SSC · 103802-104716 · 915 bp

Back to Campeiostachys

Pi 0.00437

MarkerSeek score 49.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 915
variable_sites: 6
pi: 0.00437
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAAGAGGGAATTGTGCGGAT	GATGCTCATTACCGCTGGAA	50.0	50.0	58.0	58.0	1788-1793 bp	1.0	79.9
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	GATGCTCATTACCGCTGGAA	55.0	50.0	58.1	58.0	1099-1104 bp	1.0	79.7
ndhF-rpl32_p3	CATTCCCACGAGTTCCCAAA	GATGCTCATTACCGCTGGAA	50.0	50.0	58.1	58.0	1517-1522 bp	1.0	79.5
ndhF-rpl32_p4	GAAGAGGGAATTGTGCGGAT	ACCCTTTGGTTTTGGATGCT	50.0	45.0	58.0	57.9	1802-1807 bp	1.0	79.4
ndhF-rpl32_p5	AACACATTCCCACGAGTTCC	GATGCTCATTACCGCTGGAA	50.0	50.0	58.1	58.0	1521-1526 bp	1.0	79.4