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rbcL-psaI

LSC · 55891-57532 · 1642 bp

Back to Campeiostachys

Pi 0.00305

MarkerSeek score 46.00

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1642
variable_sites: 7
pi: 0.00305
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CCGAAATCTTTGGGGACGAT	AACTCGCTATACCTGGCTCT	50.0	50.0	58.0	57.9	2159-2165 bp	1.0	79.7
rbcL-psaI_p2	TCTTCACATTCACCGTGCAA	AACTCGCTATACCTGGCTCT	45.0	50.0	58.0	57.9	2461-2467 bp	1.0	79.7
rbcL-psaI_p3	TTCTTCACATTCACCGTGCA	AACTCGCTATACCTGGCTCT	45.0	50.0	58.0	57.9	2462-2468 bp	1.0	79.7
rbcL-psaI_p4	CCGAAATCTTTGGGGACGAT	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	1973-1974 bp	1.0	79.4
rbcL-psaI_p5	TCTTCACATTCACCGTGCAA	GTCATTGCAATTGCCGGAAA	45.0	45.0	58.0	57.9	2275-2276 bp	1.0	79.4