Hotspot

trnS-GGA-rps4

LSC · 44562-44867 · 306 bp

Back to Campeiostachys

Pi 0.00218
MarkerSeek score 49.40
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
LSC
length_bp
306
variable_sites
1
pi
0.00218
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 ATGTAGGAGAGATGGCCGAG AGATAGGAAGTGGGTCGGTC 55.0 55.0 58.4 58.2 462-468 bp 1.0 76.4
trnS-GGA-rps4_p2 ATGTAGGAGAGATGGCCGAG ATAGGAAGTGGGTCGGTCTC 55.0 55.0 58.4 58.2 460-466 bp 1.0 76.4
trnS-GGA-rps4_p3 ATGTAGGAGAGATGGCCGAG GATAGGAAGTGGGTCGGTCT 55.0 55.0 58.4 58.2 461-467 bp 1.0 76.4
trnS-GGA-rps4_p4 ATGTAGGAGAGATGGCCGAG GAAGTGGGTCGGTCTCAAAA 55.0 50.0 58.4 57.8 456-462 bp 1.0 76.2
trnS-GGA-rps4_p5 TTCGAATCCCTCTCTTTCCG ATAGGAAGTGGGTCGGTCTC 50.0 55.0 57.0 58.2 388-394 bp 1.0 72.0