Hotspot

trnfM-CAU-trnT-GGU

LSC · 12683-14652 · 1970 bp

Back to Campeiostachys

Pi 0.00102

MarkerSeek score 58.50

Primer available yes

Label trnfM-CAU-trnT-GGU

Feature Metadata

label_name: trnfM-CAU-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1970
variable_sites: 3
pi: 0.00102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnT-GGU_p1	CCTCAAGGTTATGAGCCTCG	TGAACCGATGACTTATGCCT	55.0	45.0	57.8	56.6	2065-2066 bp	1.0	67.3
trnfM-CAU-trnT-GGU_p2	CCTCGTGAGCTACCAAACTG	TGAACCGATGACTTATGCCT	55.0	45.0	58.3	56.6	2050-2051 bp	1.0	66.9
trnfM-CAU-trnT-GGU_p3	CCTCAAGGTTATGAGCCTCG	TGACTTACGTCTTAGCACGAG	55.0	47.6	57.8	57.5	2125-2127 bp	1.0	66.6
trnfM-CAU-trnT-GGU_p4	CCTCAAGGTTATGAGCCTCG	CCGATGACTTATGCCTTACCA	55.0	47.6	57.8	57.5	2061-2062 bp	1.0	66.6
trnfM-CAU-trnT-GGU_p5	CCTCAAGGTTATGAGCCTCG	ACCGATGACTTATGCCTTACC	55.0	47.6	57.8	57.5	2062-2063 bp	1.0	66.5