Hotspot

ccsA-ndhD

SSC · 137151-137423 · 273 bp

Back to Campanula

Pi 0.059

MarkerSeek score 67.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 273
variable_sites: 35
pi: 0.059
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGGTATTCTTTCGGGAGCAG	AACGAAATGGGTGGAGTAGC	55.0	50.0	58.0	57.9	967-1014 bp	1.0	86.4
ccsA-ndhD_p2	ACCCAAGGCTTTGCTACTTC	AACGAAATGGGTGGAGTAGC	50.0	50.0	58.1	57.9	1335-1382 bp	1.0	85.1
ccsA-ndhD_p3	AGTTATGCGGCTCTTCTGTG	AACGAAATGGGTGGAGTAGC	50.0	50.0	58.0	57.9	1224-1246 bp	0.8	77.0
ccsA-ndhD_p4	AGTTATGCGGCTCTTCTGTG	CCTTTTCGATGGCTTCCCTT	50.0	50.0	58.0	58.2	1169-1191 bp	0.8	76.6
ccsA-ndhD_p5	AAATTCTGCAATTGTGGCCG	AACGAAATGGGTGGAGTAGC	45.0	50.0	57.9	57.9	823-870 bp	0.8	73.0