Hotspot

rpl32-trnL-UAG

SSC · 135437-136019 · 583 bp

Back to Campanula

Pi 0.0487

MarkerSeek score 73.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 583
variable_sites: 65
pi: 0.0487
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTAGGCCAATTCTTACCGCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1130-1147 bp	0.8	75.2
rpl32-trnL-UAG_p2	TGTAGGCCAATTCTTACCGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1131-1148 bp	0.8	75.2
rpl32-trnL-UAG_p3	GTAGGCCAATTCTTACCGCA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1131-1148 bp	0.8	75.2
rpl32-trnL-UAG_p4	TGTAGGCCAATTCTTACCGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1132-1149 bp	0.8	75.2
rpl32-trnL-UAG_p5	GTAGGCCAATTCTTACCGCA	CACAGAAGAGCCGCATAACT	50.0	50.0	58.0	58.0	1748-1750 bp	0.6	63.6