Hotspot

ndhB-trnM-CAU

LSC · 93840-94329 · 490 bp

Back to Campanula

Pi 0.116
MarkerSeek score 72.80
Primer available yes
Label ndhB-trnM-CAU

Feature Metadata

label_name
ndhB-trnM-CAU
feature_type
igs
region
LSC
length_bp
490
variable_sites
138
pi
0.116
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnM-CAU_p1 TTGCTCAGGATACCCTTTTT CCTGTTAAGCATCCATGGCT 40.0 50.0 54.5 57.9 595-1066 bp 1.0 62.3
ndhB-trnM-CAU_p2 ATTGCTCAGGATACCCTTTT CCTGTTAAGCATCCATGGCT 40.0 50.0 54.3 57.9 596-1067 bp 1.0 60.4
ndhB-trnM-CAU_p3 ATTGCTCAGGATACCCTTTTT CCTGTTAAGCATCCATGGCT 38.1 50.0 55.1 57.9 596-1067 bp 1.0 59.4
ndhB-trnM-CAU_p4 TTGCTCAGGATACCCTTTTT TGGCGAATTCGTAGGTTCAA 40.0 45.0 54.5 57.5 548-1019 bp 1.0 59.4
ndhB-trnM-CAU_p5 TTGCTCAGGATACCCTTTTT TTGGCGAATTCGTAGGTTCA 40.0 45.0 54.5 57.5 549-1020 bp 1.0 59.4