Hotspot

trnD-GUC-psbJ

LSC · 56607-58294 · 1688 bp

Back to Campanula

Pi 0.15

MarkerSeek score 74.90

Primer available yes

Label trnD-GUC-psbJ

Feature Metadata

label_name: trnD-GUC-psbJ
feature_type: igs
region: LSC
length_bp: 1688
variable_sites: 586
pi: 0.15
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbJ_p1	CATCCCCCATTCCCAGATTG	GCGAAAAAGAAAGGGCTTCC	55.0	50.0	58.0	57.9	2343-2640 bp	0.8	79.0
trnD-GUC-psbJ_p2	CATCCCCCATTCCCAGATTG	TTCCTTCCCCTTTTTCGCAA	55.0	45.0	58.0	57.9	2327-2624 bp	0.8	78.9
trnD-GUC-psbJ_p3	CATCCCCCATTCCCAGATTG	CTTTTTCGCAACGCTTCCTT	55.0	45.0	58.0	57.9	2318-2615 bp	0.8	78.6
trnD-GUC-psbJ_p4	ATCCCCCATTCCCAGATTGA	GCGAAAAAGAAAGGGCTTCC	50.0	50.0	58.1	57.9	2342-2639 bp	0.8	78.5
trnD-GUC-psbJ_p5	TCCCCCATTCCCAGATTGAT	GCGAAAAAGAAAGGGCTTCC	50.0	50.0	58.1	57.9	2341-2638 bp	0.8	78.5