Hotspot

trnE-UUC-trnY-GUA

LSC · 56290-56348 · 59 bp

Back to Campanula

Pi 0.0508

MarkerSeek score 67.20

Primer available yes

Label trnE-UUC-trnY-GUA

Feature Metadata

label_name: trnE-UUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 7
pi: 0.0508
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnY-GUA_p1	TCTCTTTCAAGGAGGCAACG	CCAGCGTAGACATATTGCCA	50.0	50.0	57.8	57.8	167 bp	1.0	80.1
trnE-UUC-trnY-GUA_p2	TCTCTTTCAAGGAGGCAACG	ACCAGCGTAGACATATTGCC	50.0	50.0	57.8	57.8	168 bp	1.0	80.1
trnE-UUC-trnY-GUA_p3	TCTCTTTCAAGGAGGCAACG	CGAATTTACAGTCCGTCCCC	50.0	55.0	57.8	58.4	146 bp	1.0	79.7
trnE-UUC-trnY-GUA_p4	TCTCTTTCAAGGAGGCAACG	CCATCGCATGGTAGATTGGT	50.0	50.0	57.8	57.7	214 bp	1.0	79.2
trnE-UUC-trnY-GUA_p5	TCGTCTAGTGGTTCAGGACA	CCAGCGTAGACATATTGCCA	50.0	50.0	57.7	57.8	189 bp	1.0	78.8