Hotspot

trnV-UAC-trnM-CAU

LSC · 44366-44561 · 196 bp

Back to Campanula

Pi 0.0469

MarkerSeek score 68.80

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 196
variable_sites: 21
pi: 0.0469
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	TGTGCATTGGGCTCTTTCAT	GATCTGAAAGTCGATCCGGG	45.0	55.0	58.1	57.9	1434-1442 bp	1.0	84.3
trnV-UAC-trnM-CAU_p2	TTTTTGAGGCCCTTCGACTC	TCTAAAAACCATTGCGGGCT	50.0	45.0	58.1	58.1	1086-1094 bp	0.6	69.3
trnV-UAC-trnM-CAU_p3	TGTGCATTGGGCTCTTTCAT	TCTAAAAACCATTGCGGGCT	45.0	45.0	58.1	58.1	1355-1363 bp	0.6	68.8
trnV-UAC-trnM-CAU_p4	TGTGCATTGGGCTCTTTCAT	AAACCATTGCGGGCTTTAGA	45.0	45.0	58.1	58.1	1358 bp	0.2	46.8
trnV-UAC-trnM-CAU_p5	TTTTTGAGGCCCTTCGACTC	AAACCATTGCGGGCTTTAGA	50.0	45.0	58.1	58.1	1089 bp	0.2	46.5