Hotspot

psbB-trnV-GAC

LSC · 28668-29413 · 746 bp

Back to Campanula

Pi 0.0559

MarkerSeek score 75.20

Primer available yes

Label psbB-trnV-GAC

Feature Metadata

label_name: psbB-trnV-GAC
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 88
pi: 0.0559
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-trnV-GAC_p1	ACACCCATATTCCAGGACCA	ACCTTGTCTATTTGGCGTCC	50.0	50.0	58.0	57.9	1868 bp	0.6	63.3
psbB-trnV-GAC_p2	TGCACCAAAACCAAAGCAAG	ACCTTGTCTATTTGGCGTCC	45.0	50.0	58.0	57.9	1828 bp	0.6	63.3
psbB-trnV-GAC_p3	CCCCGCGAGAAATAAATGGA	ACCTTGTCTATTTGGCGTCC	50.0	50.0	58.0	57.9	1804 bp	0.6	63.2
psbB-trnV-GAC_p4	ACACCCATATTCCAGGACCA	CCCCCAAATCCGTTTACCAT	50.0	50.0	58.0	57.8	1808 bp	0.6	63.0
psbB-trnV-GAC_p5	CCAACCACCCCATGAATTGA	ACCTTGTCTATTTGGCGTCC	50.0	50.0	58.1	57.9	1597 bp	0.6	62.9