Hotspot

rpl2-rps19

LSC · 16642-16700 · 59 bp

Back to Campanula

Pi 0.0475

MarkerSeek score 71.40

Primer available yes

Label rpl2-rps19

Feature Metadata

label_name: rpl2-rps19
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 6
pi: 0.0475
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rps19_p1	TGGGTAGAGCCGGATCTAAG	TCTTCAGGCTAGTACCCTCG	55.0	55.0	58.0	57.9	881-885 bp	1.0	83.0
rpl2-rps19_p2	TGGGTAGAGCCGGATCTAAG	CTAGAAGGTGGCGAGCATTT	55.0	50.0	58.0	58.0	818 bp	0.2	47.7
rpl2-rps19_p3	GATGACGGAAGGGAAAGACC	CTAGAAGGTGGCGAGCATTT	55.0	50.0	58.0	58.0	1257 bp	0.2	47.6
rpl2-rps19_p4	CCAGATGACGGAAGGGAAAG	CTAGAAGGTGGCGAGCATTT	55.0	50.0	58.0	58.0	1260 bp	0.2	47.5
rpl2-rps19_p5	GATGACGGAAGGGAAAGACC	TCTTCAGGCTAGTACCCTCG	55.0	55.0	58.0	57.9	1320 bp	0.2	47.4