Hotspot

atpF-atpH

LSC · 13120-13540 · 421 bp

Back to Campanula

Pi 0.038

MarkerSeek score 68.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 36
pi: 0.038
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GTACAGCTGGTTGGATCCTG	GGGTCGGAACAAAGAAAGGT	55.0	50.0	58.0	58.0	1092 bp	0.2	47.6
atpF-atpH_p2	GTACAGCTGGTTGGATCCTG	TGGGTCGGAACAAAGAAAGG	55.0	50.0	58.0	58.0	1093 bp	0.2	47.6
atpF-atpH_p3	AGTACAGCTGGTTGGATCCT	GGGTCGGAACAAAGAAAGGT	50.0	50.0	58.0	58.0	1093 bp	0.2	47.5
atpF-atpH_p4	AGTACAGCTGGTTGGATCCT	TGGGTCGGAACAAAGAAAGG	50.0	50.0	58.0	58.0	1094 bp	0.2	47.5
atpF-atpH_p5	GTACAGCTGGTTGGATCCTG	ATTTCTGCCGCTTCCGTTAT	55.0	45.0	58.0	58.0	847 bp	0.2	47.5