Hotspot

trnR-UCU-atpA

LSC · 10128-10240 · 113 bp

Back to Campanula

Pi 0.0496

MarkerSeek score 75.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 13
pi: 0.0496
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	TTGGGTCTGCAGCTCAAATT	50.0	45.0	58.0	58.0	799-805 bp	1.0	85.0
trnR-UCU-atpA_p2	CCTAGCCTTCCAAGCTAACG	TTGGGTCTGCAGCTCAAATT	55.0	45.0	58.1	58.0	802-808 bp	1.0	84.7
trnR-UCU-atpA_p3	AGCCTTCCAAGCTAACGATG	TTGGATTTGGCGCAATTGAC	50.0	45.0	58.0	57.9	746-752 bp	1.0	84.6
trnR-UCU-atpA_p4	CCTAGCCTTCCAAGCTAACG	TTGGATTTGGCGCAATTGAC	55.0	45.0	58.1	57.9	749-755 bp	1.0	84.3
trnR-UCU-atpA_p5	AAATGCTGACTTGACGTCGA	TTGGGTCTGCAGCTCAAATT	45.0	45.0	57.9	58.0	830-836 bp	1.0	83.8