Hotspot

rps16

LSC · 49380-50502 · 1123 bp

Back to Camellia

Pi 0.00248

MarkerSeek score 48.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1123
variable_sites: 59
pi: 0.00248
unique_haplotype_count: 67
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCACTCCCCCACCTTTTGTA	CGGCTACAAATTAAAGAAATGT	50.0	31.8	58.2	53.4	1158-1197 bp	1.0	48.1
rps16_p2	TCACTCCCCCACCTTTTGTA	CGGCTACAAATTAAAGAAATGTT	50.0	30.4	58.2	54.2	1158-1197 bp	1.0	48.1
rps16_p3	CATCACTCCCCCACCTTTTG	CGGCTACAAATTAAAGAAATGT	55.0	31.8	58.5	53.4	1160-1199 bp	1.0	48.1
rps16_p4	ATACGAGTCATCACTCCCCC	CGGCTACAAATTAAAGAAATGT	55.0	31.8	58.3	53.4	1168-1207 bp	1.0	48.0
rps16_p5	ATACGAGTCATCACTCCCCC	CGGCTACAAATTAAAGAAATGTT	55.0	30.4	58.3	54.2	1168-1207 bp	1.0	48.0