Hotspot

ndhG

SSC · 8050-8580 · 531 bp

Back to Camellia

Pi 0.00377

MarkerSeek score 28.50

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 2
pi: 0.00377
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 28.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGATTTTGATTTCTAGTTCTGTTCT	ACTGGTCAGGTCAACAAAGTC	28.0	47.6	55.0	58.1	628-636 bp	0.974576	43.2
ndhG_p2	TGATTTTGATTTCTAGTTCTGTTCT	CTGGTCAGGTCAACAAAGTCA	28.0	47.6	55.0	58.1	627-635 bp	0.974576	43.2
ndhG_p3	TGATTTTGATTTCTAGTTCTGTTCT	TGGTCAGGTCAACAAAGTCA	28.0	45.0	55.0	56.9	626-634 bp	0.974576	43.2
ndhG_p4	TGATTTTGATTTCTAGTTCTGTTCT	ACTGGTCAGGTCAACAAAGT	28.0	45.0	55.0	56.9	628-636 bp	0.974576	43.2
ndhG_p5	AGTTTGATTTTGATTTCTAGTTCTGT	ACTGGTCAGGTCAACAAAGTC	26.9	47.6	55.9	58.1	632-640 bp	0.974576	43.2