Hotspot

clpP

LSC · 116423-118403 · 1981 bp

Back to Camellia

Pi 0.00151

MarkerSeek score 53.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1981
variable_sites: 50
pi: 0.00151
unique_haplotype_count: 66
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CGCGAAATCCATATCCATGC	ACCCATTGTTACGTTTCCACA	50.0	42.9	57.5	58.1	2062-2078 bp	1.0	70.6
clpP_p2	AATCACGGATTTGCGTTTCG	ACCCATTGTTACGTTTCCACA	45.0	42.9	57.8	58.1	2080-2096 bp	0.940678	69.8
clpP_p3	GCGTTTCGCGAAATCCATAT	ACCCATTGTTACGTTTCCACA	45.0	42.9	57.3	58.1	2068-2084 bp	0.991525	69.2
clpP_p4	AATCACGGATTTGCGTTTCG	CCCATTGTTACGTTTCCACA	45.0	45.0	57.8	56.3	2079-2095 bp	0.940678	64.3
clpP_p5	AATCACGGATTTGCGTTTCG	ACCCATTGTTACGTTTCCAC	45.0	45.0	57.8	56.3	2080-2096 bp	0.940678	64.3