Hotspot

trnF-GAA-ndhJ

LSC · 94647-95313 · 667 bp

Back to Camellia

Pi 0.00251

MarkerSeek score 55.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 667
variable_sites: 29
pi: 0.00251
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	735-830 bp	0.991525	85.9
trnF-GAA-ndhJ_p2	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	723-818 bp	0.991525	84.9
trnF-GAA-ndhJ_p3	ATGCATCGTGAATAGTCGGG	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.8	58.1	752-847 bp	0.991525	84.7
trnF-GAA-ndhJ_p4	TCGTGTCACCAGTTCAAGTC	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.8	58.1	697-792 bp	0.991525	84.3
trnF-GAA-ndhJ_p5	CGTGTCACCAGTTCAAGTCT	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.8	58.1	696-791 bp	0.991525	84.3