Hotspot

trnE-UUC-psbD

LSC · 76278-78647 · 2370 bp

Back to Camellia

Pi 0.00269

MarkerSeek score 66.50

Primer available yes

Label trnE-UUC-psbD

Feature Metadata

label_name: trnE-UUC-psbD
feature_type: igs
region: LSC
length_bp: 2370
variable_sites: 148
pi: 0.00269
unique_haplotype_count: 102
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-psbD_p1	TTTCGTAGTACCCTACCCCC	GAAACGGTCCCTACGTAACC	55.0	55.0	57.8	58.0	2182-2584 bp	1.0	87.5
trnE-UUC-psbD_p2	GCTGCCTCCTTGAAAGAGAG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.3	58.0	2144-2546 bp	1.0	86.7
trnE-UUC-psbD_p3	TTTCGTAGTACCCTACCCCC	GGACCAGCCTACAAAAACGA	55.0	50.0	57.8	58.1	2200-2602 bp	1.0	86.6
trnE-UUC-psbD_p4	TGTCCTGAACCACTAGACGA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.7	58.0	2123-2525 bp	1.0	86.6
trnE-UUC-psbD_p5	GCTGCCTCCTTGAAAGAGAG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.3	58.1	2162-2564 bp	1.0	85.9