Hotspot

trnS-GCU-trnR-UCU

LSC · 53443-55128 · 1686 bp

Back to Camellia

Pi 0.00387

MarkerSeek score 59.30

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1686
variable_sites: 102
pi: 0.00387
unique_haplotype_count: 71
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ATTAGCAATCCGCCGCTTTA	AGAAGACCTTTGTCCTATCCA	45.0	42.9	58.0	55.7	1734-1792 bp	1.0	61.9
trnS-GCU-trnR-UCU_p2	ATTAGCAATCCGCCGCTTTA	AGGTTTAGAAGACCTTTGTCCT	45.0	40.9	58.0	56.7	1740-1798 bp	1.0	61.6
trnS-GCU-trnR-UCU_p3	CTTTAGTCCACTCAGCCACC	AGAAGACCTTTGTCCTATCCA	55.0	42.9	57.9	55.7	1719-1777 bp	1.0	61.4
trnS-GCU-trnR-UCU_p4	CTTTAGTCCACTCAGCCACC	AGGTTTAGAAGACCTTTGTCCT	55.0	40.9	57.9	56.7	1725-1783 bp	1.0	61.2
trnS-GCU-trnR-UCU_p5	ACGGAAAGAGAGGGATTCGA	AGAAGACCTTTGTCCTATCCA	50.0	42.9	58.2	55.7	1783-1841 bp	1.0	60.9