Hotspot

rps16-trnQ-UUG

LSC · 50503-52115 · 1613 bp

Back to Camellia

Pi 0.00211

MarkerSeek score 60.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1613
variable_sites: 81
pi: 0.00211
unique_haplotype_count: 72
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAAATGAAAATGGGGCGTGG	50.0	50.0	57.9	58.0	1653-1786 bp	1.0	87.1
rps16-trnQ-UUG_p2	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1595-1728 bp	1.0	86.7
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAAATGAAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1636-1769 bp	1.0	86.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1578-1711 bp	1.0	86.1
rps16-trnQ-UUG_p5	CAACGGATCGTGTCCTTCAA	GAAATGAAAATGGGGCGTGG	50.0	50.0	58.2	58.0	1654-1787 bp	1.0	86.1