Hotspot

matK-trnK-UUU

LSC · 47878-48594 · 717 bp

Back to Camellia

Pi 0.00339

MarkerSeek score 57.90

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 717
variable_sites: 37
pi: 0.00339
unique_haplotype_count: 45
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CGAAGTCGTGTTGCTGAGAT	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.3	58.2	790-810 bp	0.991525	83.7
matK-trnK-UUU_p2	CGAAGTCGTGTTGCTGAGAT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.3	58.2	789-809 bp	0.991525	83.7
matK-trnK-UUU_p3	CGAAGTCGTGTTGCTGAGAT	GGGTTGCTAACTCAACGGTA	50.0	50.0	58.3	57.5	803-823 bp	0.991525	81.3
matK-trnK-UUU_p4	CGAAGTCGTGTTGCTGAGAT	TTCGGGTTGCTAACTCAACG	50.0	50.0	58.3	58.5	806-826 bp	0.991525	81.0
matK-trnK-UUU_p5	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.2	58.2	841-861 bp	0.991525	80.1