Hotspot

rpl32-trnL-UAG

SSC · 112838-113506 · 669 bp

Back to Camelina

Pi 0.0101
MarkerSeek score 50.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
669
variable_sites
17
pi
0.0101
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGATATTGGACAGCGTTGA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.7 57.9 799-820 bp 1.0 78.4
rpl32-trnL-UAG_p2 AGGGATATTGGACAGCGTTG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.7 57.9 800-821 bp 1.0 78.3
rpl32-trnL-UAG_p3 GGGATATTGGACAGCGTTGA AGCAGCGTGTCTACCAATTT 50.0 45.0 57.7 57.8 792-813 bp 1.0 77.9
rpl32-trnL-UAG_p4 AGGGATATTGGACAGCGTTG AGCAGCGTGTCTACCAATTT 50.0 45.0 57.7 57.8 793-814 bp 1.0 77.9
rpl32-trnL-UAG_p5 GGGATATTGGACAGCGTTGA CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.7 57.8 797-818 bp 1.0 77.8