Hotspot

psaJ-rpl33

LSC · 66168-66603 · 436 bp

Back to Camelina

Pi 0.0148

MarkerSeek score 57.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 436
variable_sites: 15
pi: 0.0148
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTCGGTTCGTTAGCAGGTTT	ATATCTGGAAATTCCCGCCG	45.0	50.0	57.8	57.8	603-616 bp	1.0	79.8
psaJ-rpl33_p2	ATGGTTCGGTTCGTTAGCAG	ATATCTGGAAATTCCCGCCG	50.0	50.0	58.3	57.8	607-620 bp	1.0	79.5
psaJ-rpl33_p3	TTCGGTTCGTTAGCAGGTTT	AATTCCCGCCGATTCTTTCT	45.0	45.0	57.8	57.6	594-607 bp	1.0	78.0
psaJ-rpl33_p4	TTCGGTTCGTTAGCAGGTTT	ATTCCCGCCGATTCTTTCTT	45.0	45.0	57.8	57.6	593-606 bp	1.0	78.0
psaJ-rpl33_p5	GTTCGGTTCGTTAGCAGGTT	ATATCTGGAAATTCCCGCCG	50.0	50.0	58.5	57.8	604-617 bp	1.0	77.8