Hotspot

trnP-UGG-psaJ

LSC · 65654-66038 · 385 bp

Back to Camelina

Pi 0.0112

MarkerSeek score 42.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 10
pi: 0.0112
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	AAACCTGCTAACGAACCGAA	45.0	45.0	57.9	57.8	488-494 bp	1.0	79.8
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	CTGCTAACGAACCGAACCAT	45.0	50.0	57.9	58.3	484-490 bp	1.0	79.5
trnP-UGG-psaJ_p3	CAAAACAAACGCGCTACCAA	AAACCTGCTAACGAACCGAA	45.0	45.0	58.2	57.8	489-495 bp	1.0	79.3
trnP-UGG-psaJ_p4	TTGTACCCAAAACAAACGCG	AAACCTGCTAACGAACCGAA	45.0	45.0	57.8	57.8	496-502 bp	1.0	79.1
trnP-UGG-psaJ_p5	CAAAACAAACGCGCTACCAA	CTGCTAACGAACCGAACCAT	45.0	50.0	58.2	58.3	485-491 bp	1.0	79.0