Hotspot

atpB-rbcL

LSC · 53375-54173 · 799 bp

Back to Camelina

Pi 0.00489

MarkerSeek score 58.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 799
variable_sites: 11
pi: 0.00489
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	943-945 bp	1.0	73.0
atpB-rbcL_p2	CGAAACCTCTGGATCCGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	885-887 bp	1.0	72.9
atpB-rbcL_p3	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	942-944 bp	1.0	72.7
atpB-rbcL_p4	CGAAACCTCTGGATCCGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	884-886 bp	1.0	72.6
atpB-rbcL_p5	TCCAGTACCGGACCAATGAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	941-943 bp	1.0	72.4