Hotspot

trnL-UAA-trnF-GAA

LSC · 46058-47372 · 1315 bp

Back to Camelina

Pi 0.0327

MarkerSeek score 60.20

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 1315
variable_sites: 61
pi: 0.0327
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.53
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	565-1405 bp	1.0	85.1
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	562-1402 bp	1.0	81.9
trnL-UAA-trnF-GAA_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	554-1394 bp	1.0	81.3
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	527-1367 bp	1.0	81.0
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	537-1377 bp	1.0	80.9