Hotspot

trnT-GGU-psbD

LSC · 30209-31237 · 1029 bp

Back to Camelina

Pi 0.00435

MarkerSeek score 56.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 12
pi: 0.00435
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1178-1187 bp	1.0	80.6
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1181-1190 bp	1.0	80.5
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.3	58.0	1155-1164 bp	1.0	78.9
trnT-GGU-psbD_p4	TGGCCCTTTTAACTCAGTGG	ACCTACAAAAACGAAGCGGT	50.0	45.0	57.7	58.0	1194-1203 bp	1.0	78.8
trnT-GGU-psbD_p5	GGCCCTTTTAACTCAGTGGT	ACCTACAAAAACGAAGCGGT	50.0	45.0	57.7	58.0	1193-1202 bp	1.0	78.8