Hotspot

petN-psbM

LSC · 26998-27532 · 535 bp

Back to Camelina

Pi 0.00427

MarkerSeek score 53.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 535
variable_sites: 6
pi: 0.00427
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCGTAGTGTGGGGAAGAAGT	GCACTCTTCATTCTCGTTCCT	50.0	47.6	58.0	58.0	636-640 bp	1.0	72.7
petN-psbM_p2	CGTAGTGTGGGGAAGAAGTG	GCACTCTTCATTCTCGTTCCT	55.0	47.6	57.9	58.0	635-639 bp	1.0	72.1
petN-psbM_p3	GGGGCAGAATTCACATGGAT	GCACTCTTCATTCTCGTTCCT	50.0	47.6	57.9	58.0	711-715 bp	1.0	72.0
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	ACTGCACTCTTCATTCTCGT	50.0	45.0	58.0	56.9	639-643 bp	1.0	72.0
petN-psbM_p5	GGGGCAGAATTCACATGGAT	ACTGCACTCTTCATTCTCGT	50.0	45.0	57.9	56.9	714-718 bp	1.0	71.3