Hotspot

rpoB-trnC-GCA

LSC · 25084-26194 · 1111 bp

Back to Camelina

Pi 0.00919

MarkerSeek score 55.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1111
variable_sites: 23
pi: 0.00919
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCGGGTATTGTAGATGTTCCC	CCGGATTTGAACTGGGGAAA	52.4	50.0	57.9	58.1	841-1214 bp	1.0	76.0
rpoB-trnC-GCA_p2	GTTCCCTCTTTTTCATCCCCA	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.8	58.1	826-1199 bp	1.0	75.6
rpoB-trnC-GCA_p3	TGTTCCCTCTTTTTCATCCCC	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.8	58.1	827-1200 bp	1.0	75.6
rpoB-trnC-GCA_p4	CCTCTTTTTCATCCCCAAGC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.7	58.1	822-1195 bp	1.0	74.3
rpoB-trnC-GCA_p5	CCGGGTATTGTAGATGTTCCC	AAAAGGATTTGCAGTCCCCC	52.4	50.0	57.9	58.4	823-1196 bp	1.0	73.8