Hotspot

trnK-UUU-trnQ-UUG

LSC · 4325-5465 · 1141 bp

Back to Camelina

Pi 0.00618

MarkerSeek score 55.90

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1141
variable_sites: 19
pi: 0.00618
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	CTACCGCAGATTTGGACGAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1263-1836 bp	1.0	81.3
trnK-UUU-trnQ-UUG_p2	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1199-1772 bp	1.0	80.1
trnK-UUU-trnQ-UUG_p3	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1198-1771 bp	1.0	80.1
trnK-UUU-trnQ-UUG_p4	CGCAGATTTGGACGAATCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.3	58.1	1259-1832 bp	1.0	78.6
trnK-UUU-trnQ-UUG_p5	CTCTACCGCAGATTTGGACG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1265-1838 bp	1.0	77.8