Hotspot

ycf2

IRb · 88404-94571 · 6168 bp

Back to Calystegia

Pi 0.000216

MarkerSeek score 28.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6168
variable_sites: 2
pi: 0.000216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGGCTGATGATGGTACTTCG	TCTGTGAAATGGTTTCCCGG	50.0	50.0	57.4	58.1	203 bp	1.0	74.8
ycf2_p2	TGGCTGATGATGGTACTTCG	AATGGTTTCCCGGGATTCAG	50.0	50.0	57.4	57.9	196 bp	1.0	74.4
ycf2_p3	TGGCTGATGATGGTACTTCG	GTGAAATGGTTTCCCGGGAT	50.0	50.0	57.4	58.2	200 bp	1.0	74.4
ycf2_p4	TGGCTGATGATGGTACTTCG	CGGGATTCAGCCAATTGTCT	50.0	50.0	57.4	58.2	186 bp	1.0	73.7
ycf2_p5	GGCTGATGATGGTACTTCGA	TCTGTGAAATGGTTTCCCGG	50.0	50.0	57.1	58.1	202 bp	1.0	72.5