Hotspot

rpl32-trnL-UAG

SSC · 113298-114260 · 963 bp

Back to Calystegia

Pi 0.00346

MarkerSeek score 47.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 963
variable_sites: 5
pi: 0.00346
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAAGAGCTTTTGGACAGCA	TCCTAAGAGCAGCGTGTCTA	42.9	50.0	58.1	57.9	1113 bp	1.0	70.9
rpl32-trnL-UAG_p2	AGAAAGAGCTTTTGGACAGCA	AGCAGCGTGTCTACCAATTT	42.9	45.0	58.1	57.8	1106 bp	1.0	70.4
rpl32-trnL-UAG_p3	AGAAAGAGCTTTTGGACAGCA	CCTAAGAGCAGCGTGTCTAC	42.9	55.0	58.1	57.8	1112 bp	1.0	70.3
rpl32-trnL-UAG_p4	AGAAAGAGCTTTTGGACAGCA	CTAAGAGCAGCGTGTCTACC	42.9	55.0	58.1	57.8	1111 bp	1.0	70.3
rpl32-trnL-UAG_p5	AGAAAGAGCTTTTGGACAGCA	GAGCAGCGTGTCTACCAATT	42.9	50.0	58.1	58.3	1107 bp	1.0	69.7